Equipment - Characterization
Analytical ultracentrifugation: ProteomeLab XL-I
Beckman Coulter ProteomeLab XL-I analytical ultracentrifuge is equipped with absorbance (wavelength range 190 – 800 nm) and interference optics and can be used for both sedimentation velocity and sedimentation equilibrium experiments. Analytical ultracentrifugation is applicable for proteins, nucleic acids, lipids, polysaccharides, viruses and nanoparticles. It has a broad applicability including determination of sample homogeneity, oligomeric state of proteins (or molecular weight, respectively) and can be used to assess aggregation of sample and to study biomolecular interactions of self- and hetero-association systems (determination of stoichiometry and affinity).
PEAQ-DSC is a differential scanning microcalorimeter with <200 µl volume of sample capillaries. DSC technique measures the heat changes that occur in the (bio)molecular sample solution during a controlled increase or decrease in temperature, on the basis of a temperature difference between the sample and the reference material. Automated version of PEAQ-DSC enables to run up to 282 samples in a row.
VP-DSC is a differential scanning microcalorimeter with 500 µl volume of sample cell for manual filling. DSC technique measures heat changes that occur in the (bio)molecular sample solution during a controlled increase or decrease in temperature, on the basis of a temperature difference between the sample and the reference material.
Differential scanning fluorimetry: Prometheus NT.48
Prometheus precisely characterizes thermal unfolding, chemical denaturation and aggregation in a single run within broad temperature range (15 - 110 °C). It is possible to measure up 48 samples in one run.
Analytical size-exclusion chromatography coupled to an array of detectors including UV-VIS spectrometer, refractometer, viscometer and static light scattering (RALS/LALS). Suitable for analysis of sample purity, oligomeric state of proteins, study of complexes and other experiments.
Dynamic light scattering : Xtal SpectroLight 600
Dynamic light scattering is used for the analysis of protein solutions, aggregates, promiscuous inhibitors, buffers, nanoparticles, polymers or other products in solution. SpectroLight 600 can measure samples in plate fromat in submicroliter droplets. This enables direct detection of particles in crystallization plates.
Dynamic (static) light scattering: DelsaMax Core
Dynamic light scattering is used for the analysis of protein solutions, aggregates, promiscuous inhibitors, buffers, nanoparticles, polymers or other products in solution. DLS system measures the polydispersity of the sample and hydrodynamic radii (size) of the particles within range of 0.4 to 5,000 nm. This is particularly beneficial in sample characterization prior to crystallization or other experiments.
Circular dichroism: Jasco J-815
CD spectrometer Jasco J-815 can measure circular dichroism, fluorescence, total fluorescence, linear dichroism, magnetic dichroism, optical rotation dispersion, and stopped flow circular dichroism, fluorescence and absorbance.
