Software for analysis

BIACORE T200 EVALUATION SOFTWARE (GE Healtcare)                             

  • kinetic and affinity evaluations of interactions measured by SPR
  • suitable for screening applications (evaluation of up to 200 concentration series in one single evaluation)
  • available ONLY on the evaluation PC


BIAEVALUATION (GE Healthcare)                                    

  • software package for presentation and evaluation of SPR data
  • evaluation of kinetic constants from sensorgram data, determination of affinity constants
  • pre-defined and user-defined interaction models
  • simulation of sensorgrams from specified models and kinetic parameters
  •  available ONLY on the evaluation PC

CONCAT (Microcal/Malvern)                                            

  • downloadable HERE (Win7 + Win10) or HERE (WinXP-Win7)
  • combines data from multiple ITC titrations into a single file 
  • available also on the evaluation PC

GUSSI (Chad Brautigam, MBR Software Fabrik)                                          

      AUC (data analyzed in SEDFIT/SEDPHAT)

      CD (data obtained on any instrument)              

      DLS (data analyzed in SEDFIT/SEDPHAT)

      DSF (currently only data from the Bio-Rad CFX-96 thermal cycler)

      ITC (data analyzed in NITPIC and SEDPHAT)

      MST (data analyzed in PALMIST)

      isotherm analysis data (AUC, SPR)

  • simple calculations related to AUC (making of isotherms; glycoproteins and membrane protein analysis)
  •  available also on the evaluation PC

MO.AFFINITY ANALYSIS (Nanotemper Technologies)

  • software for the analysis of MST data
  • determination of binding affinity (available only 1:1 binding model)
  • available ONLY on the evaluation PC


NITPIC (Chad Brautigam, MBR Software Fabrik)                                         

  • downloadable from:
  • advanced processing of ITC data
  • integration of raw data (for subsequent analysis in SEDPHAT)
  • accurate treatment of the baseline (in contrast to manual baseline adjustment in Origin or PEAQ software) 
  • available also on the evaluation PC

ORIGIN FOR ITC/DSC (Microcal/Malvern)

  • to be opened through “Origin Launcher“                                             
  • evaluation of ITC and DSC data
  • ITC models available: one site, two sites, sequential binding, competitive binding, dissociation
  • DSC models available: two state, non-two state, two state with non-zero ΔCp, dissociation with non-zero ΔCp 
  • available ONLY on the evaluation PC

PALMIST (Chad Brautigam, MBR Software Fabrik)                                     

  • downloadable from:
  • advanced analysis of MST data
  • statistically weighted fitting of isotherms, automatic confidence-interval estimates using the error-surface-projection method, unbiased isotherm outlier detection and eradication, automatic rejection of outliers in timetrace data
  • experiment planning (data simulation)
  • various fitting models (1:1, 1:2, competition, Hill)
  • available also on the evaluation PC


PEAQ-ITC (Microcal/Malvern)                                    

  • ITC data evaluation, experimental design, assessment of data quality
  • various fitting models (one set of sites, two set of sites, sequential binding sites, competitive binding, dissociation, enzyme kinetics
  • available ONLY on the evaluation PC


PR.THERMCONTROL (Nanotemper Technologies)                                           

  • investigation of thermal unfolding (DSF)
  • calculation of Tm (melting temperature) and Tagg (aggregation onset temperature)
  • available ONLY on the evaluation PC


SEDFIT (Peter Schuck, NIH)                                               


SEDPHAT (Peter Schuck, NIH)                                          

  • downloadable from:
  • analysis of AUC data (sedimentation equilibrium experiment, advanced fitting of sedimentation velocity data)
  • analysis of interacting systems (AUC, FA, ITC, SPR, spectroscopy)
  • global multi-method analysis (GMMA = analysis of data of the interacting system studied by several different techniques)
  • variety of binding models available
  • available also on the evaluation PC

SEDNTERP (Tom Laue, BITC, University of New Hampshire)                       

  • downloadable from: (v. 2) (v. 1.09)
  • prediction of partial-specific volume of proteins, solvent density and viscosity (for interpretation of AUC and DLS data)
  • Please note that the version 2 contains bugs. It is recommended to use older version 1.09 (available for Windows XP only). 
  • available also on the evaluation PC