Core Facility of Biomolecular Interactions and Crystallization

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ABOUT CF BIC:

The core facility provides services leading to the biophysical and structural characterization of biomolecules and to study of (bio)molecular interactions.  It is equipped with the instrumentation to set up crystallization conditions of biomolecules and their complexes, basic characterization of physical properties of the molecules (analytical ultracentrifugation, analytical size-exclusion chromatography, dynamic light scattering, CD spectroscopy, differential scanning calorimetry, differential scanning fluorimetry), and to study thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, bio-layer interferometry, microscale thermophoresis, analytical ultracentrifugation).


UNIQUE FEATURES:

  • Complementary techniques for molecular interactions studies

  • Detailed analysis of macromolecular properties: homogeneity, stability, oligomeric state 

  • 18 individual machines utilizing 11 principally different biophysical techniques in one facility

  • Complex crystallization services: set-up of screening and optimization plates, automatic inspection of crystals with​ UV imaging, estimation of the precipitation diagram, quality improvement of crystals, production of crystals for structural determination, etc.


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The Core Facility is part of the Czech national center of European Research Infrastructure Consortium INSTRUCT ERIC under the Czech Infrastructure for Integrative Structural Biology (CIISB) funded by the Ministry of Education, Youth and Sports of the Czech Republic. CF BIC members are actively involved in an open pan-European network Association of Resources for Biophysical Research in Europe (ARBRE). The Core Facility is also funded by INTERREG and CIISB4HEALTH projects.

        

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