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CORE FACILITY
Biomolecular Interactions and Crystallization
CEITEC Masaryk University,
Kamenice 5, 625 00 Brno
Czech Republic
+420 549 49 8166
bic@ceitec.cz
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Services
List of services provided by CF BIC. Measurements are performed by CF BIC staff after obtaining samples from users. The results are available within a week.
Services are booked via intranet system (for more information HERE)
NEW
Sample quality assessment
Sample quality service enables to characterize protein sample prior to more advanced experiments. It includes measurement of intact mass (MS), aggregation content (DLS) and temperature stability (nanoDSF).
For more details, contact CF responsible person Josef Houser.
e-mail: josef.houser@ceitec.cz
Analytical size-exclusion chromatography
Analytical size-exclusion chromatography coupled to an array of detectors including UV-VIS spectrometer, refractometer, viscometer and static light scattering (RALS/LALS). Suitable for analysis of sample purity, oligomeric state of proteins, study of complexes and other experiments.
For more details, contact CF responsible person Josef Houser.
e-mail: josef.houser@ceitec.cz
NEW
Sedimentation velocity method can be used for determination of sample dispersity, oligomeric state of proteins, the shape of the particle and it is also suitable for analysis of aggregates and a study of biomolecular interactions.
Sedimentation equilibrium method can be used for accurate determination of molecular weights of various biomolecules and the study of their interactions.
Calorimetric titration + raw data (automated data evaluation)
Calorimetric measurement of receptor-ligand interaction (Standard titration method, Single injection method) Data evaluation - by automated software data evaluation - complete thermodynamic profile determination (association constant, enthalpy, entropy, stoichiometry value) using curve fitting models: One set of binding site.
Calorimetric titration + expert data evaluation
Calorimetric measurement of receptor-ligand interaction (Standard titration method, Single injection method) Data evaluation - manually by expert - complete thermodynamic profile determination (association constant, enthalpy, entropy, stoichiometry value) using curve fitting models: One set of binding site, Two sets of binding sites, Sequential Binding model. Determination of kinetic parameters Km and kcat.
Calorimetric titration + expert data evaluation + data evaluation training AutoITC200
Calorimetric measurement of receptor-ligand interaction (Standard titration method, Single injection method) Data evaluation - manually by expert - complete thermodynamic profile determination (association constant, enthalpy, entropy, stoichiometry value) using curve fitting models: One set of binding site, Two sets of binding sites, Sequential Binding model. Determination of kinetic parameters Km and kcat. Data evaluation training.
Standard crystallization screen set-up
Set-up of crystallization screens (drop volume 200-300 nl) using commercial kits (including kits for membrane proteins, protein complexes and nucleic acids) - Sitting drop crystallization method, Crystallization under oil.
Standard crystallization Screen Set-up, Plate Storage and Inspection
Set-up of crystallization screens (drop volume 200-300 nl) using commercial kits (including kits for membrane proteins, protein complexes and nucleic acids). Sitting drop crystallization method, crystallization under oil. Storage of the screening plates for 3 months at a constant temperature 4°C or 20°C Automatic screening of crystals . Regular automatic inspection within the period of one month. UV imaging to distinguish a protein from a salt. Online access to the images. Storage of the screening plates for extended period of time (6 months) at a constant temperature 4°C or 20°C with a possibility of a demanded extra inspection
Setting-up multidimensional gradients for optimization (from up to 5 different solutions). Set-up of optimization screens - Hanging drop crystallization method, Sitting drop crystallization method, Crystallization under oil.
Customized crystallization, Plate storage and inspection
Setting-up multidimensional gradients for optimization (from up to 5 different solutions). Set-up of optimization screens - Hanging drop crystallization method, Sitting drop crystallization method, Crystallization under oil. Storage of the screening plates for 3 months at a constant temperature 4°C or 20°C. Automatic screening of crystals - Regular automatic inspection within the period of one month, UV imaging to distinguish a protein from a salt. Online access to the images 24/7 Storage of the screening plates for extended period of time (6 months) at a constant temperature 4°C or 20°C with a possibility of a demanded extra inspection.
Storage of the screening plates for 3 months at a constant temperature 4°C or 20°C. Automatic screening of crystals. Regular automatic inspection within the period of one month. UV imaging to distinguish a protein from a salt. Online access to the images 24/7. Storage of the screening plates for extended period of time (6 months) at a constant temperature 4°C or 20°C with a possibility of a demanded extra inspection.
Advanced crystallization techniques
Optimization of protein crystal production in large scale (manual setting), Membrane protein crystallization techniques, Crystallization by dialysis or using capilaries. The service price is calculated individually. Contact the responsible person for details.
Initial training for instruments in user mode (operated by users him-herself)
Initial training for instruments which are operated by users him/herself. This includes Biacore T200 (SPR), multichannel SPR imaging system, Octet RED96e (BLI), SpectroLight 600 and DelsaMax Core (DLS), Jasco J-815 (CD), VP-ITC, VP-DSC, Monolith NT.115 Nano and Monolith NT.115 Pico (MST), Prometheus (DSF), microdeposition system for biosensor arrays and gradient incubator for optimizing protein crystallization temperature.
Training is performed by CF BIC staff - responsible person for each instrument. Without this training users cannot operate the instruments on their own.
Service includes consulting with specialist, expert data evaluation, advanced experimental design or personal user support during measurement.
Material (consumables)
While basic consumables are provided by Core facility for free, specialized material may be ordered. This includes SPR and BLI sensors, MST capillaries and fluorescent dyes or material for protein crystallization. For more details, contact CF staff.
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