SEC-MALS: OmniSEC

Technology / Methodology:
​Interactions and characterization of biomolecules

Location:
CEITEC MU,  building C04, room 2.23

Research group:
CF: Core Facility Biomolecular Interaction and Crystallography

PROFILE CARD SERVICE MODE​

Size exclusion chromatography is a method that separates molecules based on their size. It is based on a different retention of the molecules on a column with inert filling at a steady flow. When coupled to an array of detectors, it enables also a detailed analysis of composition of individual elution peaks. In the case of OmniSEC instrument, the detected features include refractive index, UV-VIS spectrum, static light scattering and intrinsic viscosity. This enables analysis of various types of samples, such as proteins and their complexes, nucleic acids, polysaccharides and nanoparticles. High pressure chromatography (1-20 MPa) ensures good separation of individual species, e.g. protein oligomers.

Features:

• Autosampler for up to 2x96 samples in vials or microtiter plates
• 1-300 µl injection volume
• Temperature range: 20 - 65 °C (column and detectors), 4 - 60 °C (autosampler)
• Differential refractometer with ±2.5 x 10^-4 RIU dynamic range
• Diode-array-based UV/Vis spectrometer with a range of 190 - 900 nm
• Two light scattering detectors (RALS at 90° angle, LALS at 7° angle) for accurate molecular mass determination over a broad range (200 - 10⁷ g/mol)
• 4-capillary Wheatstone bridge viscometer

The instrument is operated by CF BIC staff mainly. User mode upon discussion.

For more details, please contact responsible person:

​Monika Kubíčková​

e-mail: monika.kubickova@ceitec.cz
phone: +420 549 49 25 27
​office : 3.39/C04