Standard crystallization screen set-up, Plate storage and inspection

Technology/ Methodology:
Crystallization of biomolecules

Research group:
CF: Core Facility Biomolecular Interaction and Crystallization

Set-up of crystallization screens (drop volume 200-300 nl) using commercial kits (including kits for membrane proteins, protein complexes and nucleic acids). Sitting drop crystallization method, crystallization under oil.

Storage of the screening plates for 3 months at a constant temperature 4°C or 20°C Automatic screening of crystals . Regular automatic inspection within the period of one month. UV imaging to distinguish a protein from a salt. Online access to the images. Storage of the screening plates for extended period of time (6 months) at a constant temperature 4°C or 20°C with a possibility of a demanded extra inspection.

Crystallization screens available

Screen	Type	Info materials
Qiagen - Nextal
Classics Suite	B	*
Classics II Suite	B	*
Classics Lite Suite	B	*
PACT Suite	B	*
AmSO4 Suite		*
ComPAS Suite		*
MPD Suite		*
PEGs Suite		*
PEGs II Suite		*
pH Clear Suite		*
pH Clear II Suite		*
Protein Complex Suite		*
Cryos Suite	S	*
Anions Suite		*
Cations Suite		*

Molecular Dimensions
Clear Strategy I	B
Clear Strategy II	B
JCSG-plus	B
SG1 (not available now)	B	*
Structure I + II	B	*
Midas		*
Morpheus		*
Morpheus II		*
PGA		*
The BCS Screen
Helix	N	*
Stura Footprint + MacroSol	S	*
MemStart + MemSys	M	*
MemAdvantage	M	*
MemMeso	M	*
Calixar 2.0 additive kit	M, S, (24)	*
MemMagic Bicelle Screen kit	M, S, (4)	*
Angstrom Additive Screen	S	*
Hippocrates Additive Screen	S, (48)
Multi Xtal Screen	S, (48)	*
Small molecule	S