Robotics for protein crystallization
Technology / Methodology:
Crystallization of biomolecules
Location:
CEITEC MU, building C04, room 1S026
Research group:
CF: Core Facility Biomolecular Interaction and Crystallography
High-throughput protein crystallisation robotics with pipettors in microlitre and nanolitre volumes, robotics for manipulation with multiwell plates and needed SW and HW.
Crystallization screens available
| Screen | Type | Info materials |
| Qiagen - Nextal | ||
| Classics Suite | B |
 *
|
| Classics II Suite | B |
*
|
| Classics Lite Suite | B |
*
|
| PACT Suite | B |
*
|
| AmSO4 Suite |
|
*
|
| ComPAS Suite |
|
*
|
| MPD Suite |
|
*
|
| PEGs Suite |
|
*
|
| PEGs II Suite |
|
*
|
| pH Clear Suite |
|
*
|
| pH Clear II Suite |
|
*
|
| Protein Complex Suite |
|
*
|
| Cryos Suite | S |
*
|
| Anions Suite |
|
*
|
| Cations Suite |
|
*
|
| Molecular Dimensions | ||
| Clear Strategy I | B |
|
| Clear Strategy II | B |
|
| JCSG-plus | B |
|
| SG1 (not available now) | B |
*
|
| Structure I + II | B |
*
|
| Midas |
|
*
|
| Morpheus |
|
*
|
| Morpheus II |
|
*
|
| PGA |
|
*
|
| The BCS Screen |
|
|
| Helix | N |
*
|
| Stura Footprint + MacroSol | S |
*
|
| MemStart + MemSys | M |
*
|
| MemAdvantage | M |
*
|
| MemMeso | M |
*
|
| Calixar 2.0 additive kit | M, S, (24) |
*
|
| MemMagic Bicelle Screen kit | M, S, (4) |
*
|
| Angstrom Additive Screen | S |
*
|
| Hippocrates Additive Screen | S, (48) |
|
| Multi Xtal Screen | S, (48) |
*
|
| Small molecule | S |
|
| Hampton Research | ||
| Natrix 1 + 2 | N |
*
|
| Additive Screen | S |
*
|
| Detergent Screen | S |
*
|
| Nucleic Acid Mini Screen | N, (24) |
*
|
| Ionic Liquid Screen | S, (24) |
 *
|
B - basic screen, M - designed for membrane proteins, N - designed for nucleic acids,
S - screen for special purposes, (XX) - non-96well screen (number of conditions),
* - screen behind its warranty period, exact concentrations may differ from manufacturer-given values
Heavy-atom screens available
| Screen | Conditions | Info materials |
| Hampton Research |
|
|
| Heavy atom screen Pt | 12 |
|
| Heavy atom screen Hg | 15 | |
| Heavy atom screen M1 | 19 | |
| Heavy atom screen M2 | 19 |
| Jena Biosciences |
|
|
| JBS Magic triangle | 1 |
|
| Lanthanide phasing kit | 10 |
|
Individual compounds available as single reagents in powder or stock solutions.
Available services:
Standard crystallization screen set-up
Standatd crystallization screen set-up, Plate storage and inspections
Customized crystallization, Plate storage and inspection
Advanced crystallization techniques
For more details, please contact responsible person:
Josef Houser
e-mail: josef.houser@ceitec.cz
phone: +420 549 49 25 26
office : 3.39/C04
Jana Kosourová
e-mail: jana.kosourova@ceitec.cz
phone: +420 549 49 83 14
office : 3.39/C04
