Calorimetric titration + expert data evaluation
Technology / Methodology:
Biomolecular characterization and interaction
Research group:
CF: Core Facility Biomolecular Interaction and Crystalization
Calorimetric measurement of receptor-ligand interaction (Standard titration method, Single injection method) Data evaluation - manually by expert - complete thermodynamic profile determination (association constant, enthalpy, entropy, stoichiometry value) using curve fitting models: One set of binding site, Two sets of binding sites, Sequential Binding model. Determination of kinetic parameters Km and kcat.
Associated instrument:
Auto-iTC200: Automated calorimetric system
For more details, please contact responsible person:
Jitka Holková
e-mail: jitka.holkova@ceitec.cz
phone: +420 549 49 25 27
office: 3.39/A4
