Analytical Ultracentrifugation

Technology / Methodology:
Interactions and characterization of biomolecules

Location:
CEITEC MU,  building C04, room 2.17

Research group:
CF: Core Facility Biomolecular Interaction and Crystallography

PROFILE CARD (OPTIMA) PROFILE CARD (PROTEOMELAB) SERVICE MODE

Beckman Coulter ProteomeLab XL-I and Optima analytical ultracentrifuges are equipped with absorbance (wavelength range 190 – 800 nm) and interference optics and can be used for both sedimentation velocity and sedimentation equilibrium experiments.

Analytical ultracentrifugation is applicable for proteins, nucleic acids, lipids, polysaccharides, viruses and nanoparticles. It has a broad applicability including determination of sample homogeneity, oligomeric state of proteins (or molecular weight, respectively) and can be used to assess aggregation of sample and to study biomolecular interactions of self- and hetero-association systems (determination of stoichiometry and affinity).

Analytical ultracentrifugation is in "service" mode - the instrument is operated by CF BIC staff.


Available services:

Sedimentation equilibrium

Sedimentation velocity


For more details, please contact responsible person:

Monika Kubíčková

e-mail: monika.kubickova@ceitec.cz
phone: +420 549 49 25 27
​office : 3.39/C04