Fluorescence spectroscopy
Technology / Methodology:
Interactions and characterization of biomolecules
Location:
CEITEC MU, building C04, room 2.18
Research group:
CF: Core Facility Biomolecular Interaction and Crystallography
The Fluorolog-QM by HORIBA is a high-performance modular spectrofluorometer designed for advanced fluorescence research. It offers exceptional sensitivity, a wide spectral range, and flexible configuration options, making it ideal for applications such as photoluminescence, quantum yield measurements, and time-resolved fluorescence. Temperature controlled compartment allows for precise conditions determination.
Specification:
Detection modes: Steady state measurements (FRET, polarization/anisotropy, etc.), TCSPC lifetimes (including anisotropy lifetimes)
Sensitivity: 35 000:1 FSD
System: all reflective
Monochromators: single excitation and single emission monochromator (focal length 350 mm)
Compartments: Temperature controlled cuvette holder/ TCSPC compartment, absorbance compartment
Light source: 75W Xenon arc lamp
Excitation range: 200 - 900 nm
Emission range: 210 - 900 nm
Spectral resolution: 0,1 nm
Detector: standard PMTs (UV-Vis)
Lifetime range: 5ps to 1s
Laser diode for TCSPC: 647 nm
Cuvettes: standard 10x10mm cuvette (3 ml), 5x5mm cuvette (500 µl), micro-cuvette (1-5µl)
