Software for analysis
BIACORE T200 EVALUATION SOFTWARE (GE Healtcare)
- kinetic and affinity evaluations of interactions measured by SPR
- suitable for screening applications (evaluation of up to 200 concentration series in one single evaluation)
- available ONLY on the evaluation PC
BIAEVALUATION (GE Healthcare)
- software package for presentation and evaluation of SPR data
- evaluation of kinetic constants from sensorgram data, determination of affinity constants
- pre-defined and user-defined interaction models
- simulation of sensorgrams from specified models and kinetic parameters
- available ONLY on the evaluation PC
GUSSI (Chad Brautigam, MBR Software Fabrik)
- downloadable from: http://biophysics.swmed.edu/MBR/software.html
- preparation of publication-quality figures from various techniques:
AUC (data analyzed in SEDFIT/SEDPHAT)
CD (data obtained on any instrument)
DLS (data analyzed in SEDFIT/SEDPHAT)
DSF (currently only data from the Bio-Rad CFX-96 thermal cycler)
ITC (data analyzed in NITPIC and SEDPHAT)
MST (data analyzed in PALMIST)
isotherm analysis data (AUC, SPR)
- simple calculations related to AUC (making of isotherms; glycoproteins and membrane protein analysis)
available also on the evaluation PC
MO.AFFINITY ANALYSIS (Nanotemper Technologies)
- software for the analysis of MST data
- determination of binding affinity (available only 1:1 binding model)
- available ONLY on the evaluation PC
NITPIC (Chad Brautigam, MBR Software Fabrik)
- downloadable from: http://biophysics.swmed.edu/MBR/software.html
- advanced processing of ITC data
- integration of raw data (for subsequent analysis in SEDPHAT)
- accurate treatment of the baseline (in contrast to manual baseline adjustment in Origin or PEAQ software)
- available also on the evaluation PC
ORIGIN FOR ITC/DSC (Microcal/Malvern)
- to be opened through “Origin Launcher“
- evaluation of ITC and DSC data
- ITC models available: one site, two sites, sequential binding, competitive binding, dissociation
- DSC models available: two state, non-two state, two state with non-zero ΔCp, dissociation with non-zero ΔCp
- available ONLY on the evaluation PC
PALMIST (Chad Brautigam, MBR Software Fabrik)
- downloadable from: http://biophysics.swmed.edu/MBR/software.html
- advanced analysis of MST data
- statistically weighted fitting of isotherms, automatic confidence-interval estimates using the error-surface-projection method, unbiased isotherm outlier detection and eradication, automatic rejection of outliers in timetrace data
- experiment planning (data simulation)
- various fitting models (1:1, 1:2, competition, Hill)
available also on the evaluation PC
PEAQ-ITC (Microcal/Malvern)
- ITC data evaluation, experimental design, assessment of data quality
- various fitting models (one set of sites, two set of sites, sequential binding sites, competitive binding, dissociation, enzyme kinetics
available ONLY on the evaluation PC
PR.THERMCONTROL (Nanotemper Technologies)
- investigation of thermal unfolding (DSF)
- calculation of Tm (melting temperature) and Tagg (aggregation onset temperature)
- available ONLY on the evaluation PC
SEDFIT (Peter Schuck, NIH)
- downloadable from: https://sedfitsedphat.nibib.nih.gov/software
- analysis of AUC data (sedimentation velocity experiment)
- available also on the evaluation PC
SEDPHAT (Peter Schuck, NIH)
- downloadable from: https://sedfitsedphat.nibib.nih.gov/software
- analysis of AUC data (sedimentation equilibrium experiment, advanced fitting of sedimentation velocity data)
- analysis of interacting systems (AUC, FA, ITC, SPR, spectroscopy)
- global multi-method analysis (GMMA = analysis of data of the interacting system studied by several different techniques)
- variety of binding models available
- available also on the evaluation PC
SEDNTERP (Tom Laue, BITC, University of New Hampshire)
- downloadable from: http://bitcwiki.sr.unh.edu (v. 2)
http://www.jphilo.mailway.com/download.htm#SEDNTERP (v. 1.09) - prediction of partial-specific volume of proteins, solvent density and viscosity (for interpretation of AUC and DLS data)
- Please note that the version 2 contains bugs. It is recommended to use older version 1.09 (available for Windows XP only).
available also on the evaluation PC
