Differential scanning calorimetry
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Technology / Methodology:
Interactions and characterization of biomolecules
Location:
CEITEC MU, building C04, room 2.19
Research group:
CF: Core Facility Biomolecular Interaction and Crystallography
MicroCal PEAQ-DSC Automated is differential scanning microcalorimeter with 325 µl volume of sample cell. DSC technique measures heat changes that occur in the (bio)molecule sample solution during a controlled increase or decrease in temperature, on the basis of a temperature difference between the sample and the reference material.
It is a valuable technique for the study of stability of samples in solution, technique providing fast and accurate determination of the transition midpoint Tm – when 50% of the biomolecules are unfolded. In addition, a complete thermodynamic profile is generated to understand the factors that affect conformation and stability - enthalpy (ΔH) of unfolding due to heat denaturation, also the change in heat capacity (ΔCp) of denaturation can be determined. DSC is a sensitive, easy-to-use technique that requires no assay development, labelling or immobilization. Filling of the cell is crucial for the accuracy. The operating temperature range is of 2 °C to 130 °C. Scanrates fall in the range of 0°C/hr to 240°C/hr in the upscan mode and 0°C/hr to -60°C/hr in the downscan mode.
Experiment at constant temperature (Isoscan) for shelf life studies or evaluating of the compound stability is also possible.
The measurement at PEAQ-DSC Automated is supposed to be performed by CF BIC staff.
Typical concentration range: 0.01 mg/ml – 10 mg/ml
For more details, please contact responsible person:
Josef Houser
e-mail: josef.houser@ceitec.cz
phone: +420 549 49 25 26
office : 3.39/C04
| 0.01 mg/mL – 10 mg/mL 1 |
