Customized crystallization, Plate storage and inspection
Technology/ Methodology:
Crystallization of biomolecules
Research group:
CF: Core Facility Biomolecular Interaction and Crystallization
Setting-up multidimensional gradients for optimization (from up to 5 different solutions). Set-up of optimization screens - Hanging drop crystallization method, Sitting drop crystallization method, Crystallization under oil.
Storage of the screening plates for 3 months at a constant temperature 4°C or 20°C. Automatic screening of crystals - Regular automatic inspection within the period of one month, UV imaging to distinguish a protein from a salt. Online access to the images 24/7 Storage of the screening plates for extended period of time (6 months) at a constant temperature 4°C or 20°C with a possibility of a demanded extra inspection.
Crystallization screens available
| Screen | Type | Info materials |
| Qiagen - Nextal | ||
| Classics Suite | B |
|
| Classics II Suite | B |
|
| Classics Lite Suite | B |
|
| PACT Suite | B |
|
| AmSO4 Suite |
|
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| ComPAS Suite |
|
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| MPD Suite |
|
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| PEGs Suite |
|
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| PEGs II Suite |
|
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| pH Clear Suite |
|
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| pH Clear II Suite |
|
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| Protein Complex Suite |
|
|
| Cryos Suite | S |
|
| Anions Suite |
|
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| Cations Suite |
|
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| Molecular Dimensions | ||
| Clear Strategy I | B |
|
| Clear Strategy II | B |
|
| JCSG-plus | B |
|
| SG1 | B |
|
| Structure I + II | B |
|
| Midas |
|
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| Morpheus |
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| Morpheus II |
|
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| PGA |
|
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| Helix | N |
|
| MemMeso | M |
|
| MemStart + MemSys | M |
|
| MemAdvantage | M |
|
| Stura Footprint + MacroSol | S |
|
| Calixar 2.0 additive kit | M, S, (24) |
|
| MemMagic Bicelle Screen kit | M, S, (4) |
|
| Hampton Research | ||
| Natrix 1 + 2 | N |
|
| Additive Screen | S |
|
| Detergent Screen | S |
|
| Nucleic Acid Mini Screen | N, (24) |
|
| Ionic Liquid Screen | S, (24) |
|
B - basic screen, M - designed for membrane proteins, N - designed for nucleic acids,
S - screen for special purposes, (XX) - non-96well screen (number of conditions)
Associated instruments:
Robotics for protein crystallization
Automated storage and visual inspection of crystal plates (4 °C and 20 °C)
For more details, please contact responsible person:
Josef Houser
e-mail: josef.houser@ceitec.cz
phone: +420 549 49 25 27
office : 3.39/C04
Jana Kosourová
e-mail: jana.kosourova@ceitec.cz
phone: +420 549 49 83 14
office : 3.38/C04
