Customized crystallization, Plate storage and inspection
Technology/ Methodology:
Crystallization of biomolecules
Research group:
CF: Core Facility Biomolecular Interaction and Crystallization
Setting-up multidimensional gradients for optimization (from up to 5 different solutions). Set-up of optimization screens - Hanging drop crystallization method, Sitting drop crystallization method, Crystallization under oil.
Storage of the screening plates for 3 months at a constant temperature 4°C or 20°C. Automatic screening of crystals - Regular automatic inspection within the period of one month, UV imaging to distinguish a protein from a salt. Online access to the images 24/7 Storage of the screening plates for extended period of time (6 months) at a constant temperature 4°C or 20°C with a possibility of a demanded extra inspection.
Crystallization screens available
Screen | Type | Info materials |
Qiagen - Nextal | ||
Classics Suite | B |
|
Classics II Suite | B |
|
Classics Lite Suite | B |
|
PACT Suite | B |
|
AmSO4 Suite |
|
|
ComPAS Suite |
|
|
MPD Suite |
|
|
PEGs Suite |
|
|
PEGs II Suite |
|
|
pH Clear Suite |
|
|
pH Clear II Suite |
|
|
Protein Complex Suite |
|
|
Cryos Suite | S |
|
Anions Suite |
|
|
Cations Suite |
|
|
Molecular Dimensions | ||
Clear Strategy I | B |
|
Clear Strategy II | B |
|
JCSG-plus | B |
|
SG1 | B |
|
Structure I + II | B |
|
Midas |
|
|
Morpheus |
|
|
Morpheus II |
|
|
PGA |
|
|
Helix | N |
|
MemMeso | M |
|
MemStart + MemSys | M |
|
MemAdvantage | M |
|
Stura Footprint + MacroSol | S |
|
Calixar 2.0 additive kit | M, S, (24) |
|
MemMagic Bicelle Screen kit | M, S, (4) |
|
Hampton Research | ||
Natrix 1 + 2 | N |
|
Additive Screen | S |
|
Detergent Screen | S |
|
Nucleic Acid Mini Screen | N, (24) |
|
Ionic Liquid Screen | S, (24) |
|
B - basic screen, M - designed for membrane proteins, N - designed for nucleic acids,
S - screen for special purposes, (XX) - non-96well screen (number of conditions)
Associated instruments:
Robotics for protein crystallization
Automated storage and visual inspection of crystal plates (4 °C and 20 °C)
For more details, please contact responsible person:
Josef Houser
e-mail: josef.houser@ceitec.cz
phone: +420 549 49 25 26
office : 3.39/A4
Jana Kosourová
e-mail: jana.kosourova@ceitec.cz
phone: +420 549 49 83 14
office : 3.39/A4