Standard crystallization screen set-up, Plate storage and inspection

Technology/ Methodology:
Crystallization of biomolecules

Research group:
CF: Core Facility Biomolecular Interaction and Crystallization

PROFILE CARD

Set-up of crystallization screens (drop volume 200-300 nl) using commercial kits (including kits for membrane proteins, protein complexes and nucleic acids). Sitting drop crystallization method, crystallization under oil.

Storage of the screening plates for 3 months at a constant temperature 4°C or 20°C Automatic screening of crystals . Regular automatic inspection within the period of one month. UV imaging to distinguish a protein from a salt. Online access to the images. Storage of the screening plates for extended period of time (6 months) at a constant temperature 4°C or 20°C with a possibility of a demanded extra inspection.

Crystallization screens available

  Screen Type Info materials
  Qiagen - Nextal
  Classics Suite B Qiagen Web page  *
  Classics II Suite B  *
  Classics Lite Suite B ​ *
  PACT Suite B  *
  AmSO4 Suite
 *
  ComPAS Suite
 *
  MPD Suite
 *
  PEGs Suite
 *
  PEGs II Suite
 *
  pH Clear Suite
 *
  pH Clear II Suite
 *
  Protein Complex Suite
 *
  Cryos Suite S  *
  Anions Suite
 *
  Cations Suite
 *
  Molecular Dimensions
  Clear Strategy I B ​ 
  Clear Strategy II B  
  JCSG-plus B  
  SG1  (not available now) B  *
  Structure I + II B  *
  Midas
*
  Morpheus
 *
  Morpheus II
 *
  PGA
 *
  The BCS Screen
 
  Helix N  *
  Stura Footprint + MacroSol S  *
  MemStart + MemSys M  *
  MemAdvantage M  *
  MemMeso M  *
  Calixar 2.0 additive kit M, S, (24) *
  MemMagic Bicelle Screen kit M, S, (4) ​ *
  Angstrom Additive Screen S  *
  Hippocrates Additive Screen S, (48)  
  Multi Xtal Screen S, (48) ​ *
  Small molecule S  
  Hampton Research
  Natrix 1 + 2 N  *
  Additive Screen S  *
  Detergent Screen S  *
  Nucleic Acid Mini Screen N, (24)  *
  Ionic Liquid Screen S, (24) ​ *

​​B - basic screen, M - designed for membrane proteins, N - designed for nucleic acids,

S - screen for special purposes, (XX) - non-96well screen (number of conditions)​,

* - screen behind its warranty period, exact concentrations may differ from manufacturer-given values

Associated instruments:

Robotics for protein crystallization​

Automated storage and visual inspection of crystal plates (4 °C and 20 °C)


For more details, please contact responsible person:

Josef Houser

e-mail: josef.houser@ceitec.cz
phone: +420 549 49 25 26
​office : 3.39/C04

Jana Kosourová

e-mail: jana.kosourova@ceitec.cz
phone: +420 549 49 83 14
​office : 3.38/C04