Information for users
Access to the Biomolecular Interactions and Crystallization Core Facility
The equipment of the Biomolecular Interactions and Crystallization Core Facility is available to both CEITEC and external users (academic and commercial). The user can access the infrastructure in the following ways:
- Instruments under direct access (USER MODE):
In user mode, the instruments are operated directly by the user himself/herself, the user reserves instrument time using planning board. The user may order a specific material for an analysis from the Core Facility, e.g. SPR chips (if needed) or use his/her own material. In the majority of cases, no additional material is needed to perform measurements.
User mode includes Biacore T200 (surface plasmon resonance), Octet RED96e (biolayer interferometry), multichannel SPR imaging system (surface plasmon resonance), DelsaMax Core (dynamic light scattering), SpectroLight 600 (dynamic light scattering), Jasco J-815 (circular dichroism spectroscopy), VP-ITC (isothermal titration calorimetry), VP-DSC (differential scanning calorimetry), TG-40 (gradient incubator for crystallization), Monolith NT.115 and Monolith NT.115 Pico (microscale thermophoresis) and Prometheus NT.48 (differential scanning fluorimetry) instruments.
If needed, the user may request assistance with the experiment or to run the instrument in service mode (see below).
- Instruments operated by Core Facility staff (SERVICE MODE):
Some instruments (Auto-iTC200 automated calorimetric system, analytical ultracentrifuge ProteomeLab XL-I, crystallization robotics) can only be operated by Core Facility staff and are offered to the user as a service.
Services can be booked via CEITEC intranet, where the user specifies the aim of the experiment, gives relevant information on the sample and his/her preference for when the analysis should be performed. The user is then contacted by a member of the CF staff in order to discuss further details.
Booking of the instruments/services:
The user reserves the instruments and services via CEITEC intranet. Users can gain access to the intranet after logging through the account of his/her institution. A simple guide for booking of devices/services on CEITEC intranet can be found on the this web pages of the core facility.
All users are instructed to secure their data after completing their experiment. Core Facility is not responsible for potential loos of the data (e.g. during hard drive failure). Core Facility staff regulary (every 3 months) backs up the data to the university cloud but cannot ensure day to day back up.
Location, booking hours:
Core Facility instruments are situated in buildings A4 and A35 of Masaryk University Campus in Brno, Bohunice (UKB). Detailed information on the location and the instrument time available for booking is available on our web pages.
Fees for using instruments/services of Core Facility:
Users interested in accessing the Core Facility (Czech and international researchers from universities and research institutes) can benefit from the support of the research infrastructure CIISB, funded by the Ministry of Education, Youth and Sports of the Czech Republic.