Služby
Seznam služeb nabízených sdílenou laboratoří Interakce a krystalizace biomolekul. Měření je prováděno zaměstnanci laboratoře po obdržení vzorků od uživatele. Výsledky měření obdrží uživatel běžně do týdne od uskutečnění měření.
Služby se rezervují pomocí plánovací tabule v záložce požadavky (ZDE)
Biomolecular characterization and interaction:
Analytical SEC-MALS
Analytical size-exclusion chromatography
Analytical size-exclusion chromatography coupled to an array of detectors including UV-VIS spectrometer, refractometer, viscometer and static light scattering (RALS/LALS). Suitable for analysis of sample purity, oligomeric state of proteins, study of complexes and other experiments.
For more details, contact CF responsible person Josef Houser.
e-mail: josef.houser@ceitec.cz
Sedimentation velocity method can be used for determination of sample dispersity, oligomeric state of proteins, the shape of the particle and it is also suitable for analysis of aggregates and a study of biomolecular interactions.
Metoda sedimentační rovnováhy se používá pro přesné určení molekulární hmotnosti biomolekul a pro studium jejich interakcí.
Biological Small-angle X-ray scattering
SAXS is used for the determination of the integral structural parameters, shape reconstruction, determination of the oligomeric state in solution, unraveling of the quaternary structure of protein complexes, and modeling of molecular flexibility. CF BIC offers SAXS data acquisition and SAXS data analysis services.
Contact the CF responsible person Tomáš Klumpler for details.
Calorimetric measurement of receptor-ligand interaction (Standard titration method, Single injection method) Data evaluation - by automated software or manually by expert - complete thermodynamic profile determination (association constant, enthalpy, entropy, stoichiometry value) using curve fitting models: One set of binding site, Two sets of binding sites, Sequential Binding model. Determination of kinetic parameters Km and kcat.
Differential scanning calorimetry
PEAQ-DSC is differential scanning microcalorimeter with <200 µl volume of sample capillaries. DSC technique measures the heat changes that occur in the (bio)molecule sample solution during a controlled increase or decrease in temperature, on the basis of a temperature difference between the sample and the reference material. Automated version of PEAQ-DSC enables to run up to 282 samples in a row unattended.
AUTO-PEAQ
DSC
Sample quality assessment
Sample quality service enables to characterize the protein sample prior to more advanced experiments. It includes measurement of intact mass (MS, in collaboration with CF Proteomics), aggregation content (DLS), and temperature stability (nanoDSF).
MS
nanoDSF
DLS
Standardní nasazení krystalizačního screenu
Nasazení krystalizačního screenu (velikost kapky 200-300 nl) za použití komerčně dostupných kitů (včetně kitů pro krystalizaci membránových proteinů, proteinových komplexů a nukleových kyselin). Standardně používané krystalizační metody - metoda sedící kapky, krystalizace pod olejem.
Optimization of protein crystal production in large scale (manual setting), Membrane protein crystallization techniques, Crystallization by dialysis or using capilaries. The service price is calculated individually. Contact the responsible person for details.
Using a home X-ray source, the diffraction of crystals can be measured. Various modes from testing of diffraction quality up to complete data collection and structure interpretation are available. Contact the responsible person for details.
Initial training for instruments in user mode:
Školení uživatelů pro přístroje obsluhované samotnými uživately
Zaškolení uživatelů na přístrojích, které používají samotní uživatelé. Jedná se o přístoje Biacore T200 (SPR), multikanálový SPR imaging systém, DelsaMax Core (DLS), Jasco J-815 (CD), VP-ITC, VP-DSC, Monolith NT.115 Nano (MST), Prometheus (DSF), TG-40. Školení je prováděno zaměstnanci CF BIC. Bez tohoto školení nemohou uživatelé tyto přístroje používat.
Service includes consulting with specialist, expert data evaluation, advanced experimental design or personal user support during measurement.
Please, contact responsible person for details.
Material (consumables)
While basic consumables are provided by Core facility for free, specialized material can be ordered. This includes SPR and BLI sensors, MST capillaries and fluorescent dyes or material for protein crystallization. For more details, contact CF staff.
CAPILLARIES
BUFFER SCREEN
LABELING KIT