11 - 13 (14)/2/2020

Podcast available HERE

AIM OF THE WORKSHOP

This workshop should be attended by structural biologists who want to improve their skills in sample analysis and optimization for structural techniques, in particular Cryo-EM. Training will be focused on importance to provide basic protein quality control as well as buffer and storage optimization via using quick and relatively cheap techniques to save precious Cryo-EM time. The course is divided into theoretical (1 day) and practical part (2 days). Lectures are focused on theory and examples of usage of particular methods. Additionally, voluntary presentation of own posters, debate with invited speakers and tutors and tour to the company manufacturing microscopes will be included. Practical part covers sample characterization techniques (dynamic light scattering, differential scanning fluorimetry, circular dichroism and differential scanning calorimetry), basics of Cryo-EM sample preparation and data collection. Trainees are allowed to bring their own sample for basic analysis. At the end of the course, trainees should understand the relationship between sample quality and structural technique output as well as be able to apply the know-how gained in their sample preparation workflow.

TARGETED AUDIENCE

The whole course will be open to a maximum of 24 selected participants. PhD students, post-docs and early career researchers are preferred.

The plenary lectures on 11th Feb will be open to general audience after registration.

REGISTRATION

On-line registration form will be available on CEITEC webpage. Application deadline is November 29th.

TRAINEE SELECTION

Selection will be done on the basis of the candidate´s CV and motivation letter. The scientific committee will give priority to candidates who come from institution without expertise in this type of methodologies and technologies. The participants should show that the outcome of the course will be used rapidly in their projects and activities. Multiple participants from single research group or institution are disfavored.

FEES

The lectures as well as the whole course for selected participants is free of charge. 

CONFIRMED SPEAKERS AND TUTORS​

HOLGER STARK (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany)

ALEXANDER FISH (Netherlands Cancer Institute, Amsterdam, Netherlands)

DAVID HASELBACH (Research Institute of Molecular Pathology, Vienna, Austria)

STEFAN KNAUER (University of Bayreuth, Germany)

BERTRAND RAYNAL (Institute Pasteur, Paris, France)

PEGGY STOLT-BERGNER (VBCF Vienna, Austria)

ARTHUR SEDIVY (VBCF Vienna, Austria)

ZBYNĚK ZDRÁHAL (CEITEC MU, Brno, Czech Republic)

ORGANISING and SCIENTIFIC COMMITEE

CF BIC, CEITEC MU, Brno: MICHAELA WIMMEROVÁ, JOSEF HOUSER, MONIKA KUBÍČKOVÁ, EVA FUJDIAROVÁ, JANA SYKOROVÁ, MARIE POKORNÁ

CF Cryo-EM, CEITEC MU, Brno: JIŘÍ NOVÁČEK, RADKA DOPITOVÁ, MICHAL BABIAK, ANATOLIJ FILIMONĚNKO

Institute Pasteur, Paris: PATRICK ENGLAND, BERTRAND RAYNAL

For more information, please contact us at bic@ceitec.cz