Automated calorimetric system

Technology / Methodology:
​Interactions and characterization of biomolecules

Location:
CEITEC MU,  building A4, room 2.18

Research group:
CF: Core Facility Biomolecular Interaction and Crystallization

PROFILE CARD SERVICE MODE

Fully automated isothermal calorimeter Auto-iTC200 with ultrasmall working cell (200  µl). ITC method is used for characterization of biomolecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and others. Enzyme kinetics, biological activity or the effect of molecular structure changes on binding mechanism can be also assessed. Complete thermodynamic profile of the molecular interaction in a single experiment (stoichiometry, Ka, ∆H and ∆S values) or kinetics parameters Km and kcat can be determined.

Auto-iTC200 is designed to measure the heat of binding. In a typical arrangement, the titrant, also referred as the ligand, is injected into the sample cell containing the macromolecule sample material. The calorimetric measurement can be done over a range of biologically relevant conditions (temperature, salt, pH, etc.). ITC system directly measure submilimolar to nanomolar binding constants (10^3 - 10^9 M-1). Measurement of the interactions with nanomolar to picomolar binding constants (10^9-10^12 M-1) can be measured using the competitive binding technique, the same principle can be used for low affinity interactions (10^3-10^2 M-1). The operating temperature range is of 2°C to 80°C. 

Measurement at AutoITC200 is performed exclusively by core facility staff.

Available services:

Calorimetric titration + raw data (automated data evaluation)

Calorimetric titration + expert data evaluation

Calorimetric titration + expert data evaluation + data evaluation training AutoITC200


For more details, please contact responsible person:

Jitka Holková

e-mail: jitka.holkova@ceitec.cz
phone: +420 549 49 25 27
office: 3.39/A4