Standard crystallization screen set-up, Plate storage and inspection

Technology/ Methodology:
Crystallization of biomolecules

Research group:
CF: Core Facility Biomolecular Interaction and Crystallization


Set-up of crystallization screens (drop volume 200-300 nl) using commercial kits (including kits for membrane proteins, protein complexes and nucleic acids). Sitting drop crystallization method, crystallization under oil.

Storage of the screening plates for 3 months at a constant temperature 4°C or 20°C Automatic screening of crystals . Regular automatic inspection within the period of one month. UV imaging to distinguish a protein from a salt. Online access to the images. Storage of the screening plates for extended period of time (6 months) at a constant temperature 4°C or 20°C with a possibility of a demanded extra inspection.

Crystallization screens available

  Screen Type Info materials
  Qiagen - Nextal
  Classics Suite B
  Classics II Suite B
  Classics Lite Suite B
  PACT Suite B
  AmSO4 Suite

  ComPAS Suite

  MPD Suite

  PEGs Suite

  PEGs II Suite

  pH Clear Suite

  pH Clear II Suite

  Protein Complex Suite

  Cryos Suite S
  Anions Suite

  Cations Suite

  Molecular Dimensions
  Clear Strategy I B
  Clear Strategy II B
  JCSG-plus B
  SG1 B
  Structure I + II B


  Morpheus II


  Helix N
  MemMeso M
  MemStart + MemSys M
  MemAdvantage M
  Stura Footprint + MacroSol S
  Calixar 2.0 additive kit M, S, (24)
  MemMagic Bicelle Screen kit M, S, (4)
  Hampton Research
  Natrix 1 + 2 N
  Additive Screen S
  Detergent Screen S
  Nucleic Acid Mini Screen N, (24)
  Ionic Liquid Screen S, (24)

B - basic screen, M - designed for membrane proteins, N - designed for nucleic acids,
S - screen for special purposes, (XX) - non-96well screen (number of conditions)

Associated instruments:

Robotics for protein crystallization​

Automated storage and visual inspection of crystal plates (4 °C and 20 °C)

For more details, please contact responsible person:

Josef Houser

phone: +420 549 49 25 26
​office : 3.39/A4

Jana Kosourová

phone: +420 549 49 83 14
​office : 3.39/A4