Summer school: Modern methods to study biomolecular interactions

4 - 6/9/2018

The rapid development of techniques for biomolecular interaction  analysis  results in  wide  portfolio

of methods being used - from basic yes-no detection to the sophisticated characterization of thermodynamics and kinetics of biomolecular interactions.  3-days-course will be dedicated to various theoretical and practical aspects of the interactions on a molecular level.

The course will be divided in to lecture and practical part. Series of lectures will take place on the first day, second and third day will be practical parts.

WHEN: September 4th – 6th 2018

WHERE: A35/211 (lectures), A4/218 (practicals)

For more information, please contact us at bic@ceitec.cz


PROGRAMME:

(Please note that there may be changes in the schedule)

4/9/2018 - Lectures (UKB, A35/211)

11.30-12.00     Registration

12.00-12.15     Introduction (Michaela Wimmerová, CEITEC MU, Brno)

12.15-12.40     Sample quality (Arthur Sedivy, VBCF, Vienna)

12.40-13.25     Analytical ultracentrifugation (Tom Clarke, UEA, Norwich)

13.25-14.10     Isothermal titration calorimetry (Anne Imberty, CERMAV, Grenoble)

14.10-14.45     Coffee break

14.45-15.30     Microscale thermophoresis (Arthur Sedivy, VBCF, Vienna)

15.30-16.15     Surface plasmon resonance (Franck Fieschi, IBS, Grenoble)

16.15-16.30     Short break

16.30-17.15     Nuclear magnetic resonance (Lukáš Žídek, CEITEC MU, Brno)

17.15-18.00     Electron paramagnetic resonance (Fraser Macmillan, UEA Norwich)

18.00-18.15     Discussion and closing remarks


5/9/2018 - Hands-on (UKB, A4/218) 

tutors:        Jan Komárek (AUC), Jitka Holková (ITC), Josef Houser (MST)

9.30-12.00       Practical session 1 (group 1 - AUC, group 2 - ITC, group 3 - MST)

12.00-13.00     Lunch

13.00-15.30     Practical session 2 (group 1 - ITC, group 2 - MST, group 3 - AUC)

15.30-16.00     Coffee break

16.00-18.30     Practical session 3 (group 1 - MST, group 2 - AUC, group 3 - ITC)


6/9/2018 - Data analysis (UKB, A4/118)

9.30-11.30       Data evaluation - AUC (Jan Komárek)

11.30-12.30     Lunch

12.30-14.30     Data evaluation - ITC (Jitka Holková)

14.30-15.00     Coffee break

15.00-17.00     Data evaluation - MST (Josef Houser, Jitka Holková)


7/9/2018 (valid for MU students enrolled in S2004 subject only) 

9.30                  Written exam